Journal: Mycoses

Article Title: Anti‐inflammatory effect of lanoconazole on 12‐ O ‐tetradecanoylphorbol‐13‐acetate‐ and 2,4,6‐trinitrophenyl chloride–induced skin inflammation in mice

doi: 10.1111/myc.13034

Figure Lengend Snippet: Inhibitory effect of LCZ on TPA‐induced increase in KC content, MIP‐2 content and MPO activity in the ears of mice. A 5‐mm‐diameter punch biopsy was obtained from the right ear 6 h after the treatment with acetone or TPA. (A) MPO activity in the ear biopsy was measured by fluorescence. (B, C) KC and MIP‐2 contents in the ear biopsies were measured by enzyme‐linked immunosorbent assay. Each column represents the mean ± standard error of the mean (n = 6). ** P < .01 compared with TPA alone (Dunnett's multiple‐comparison test, two‐sided); ## P < .01 compared with TPA (−) (Student's t test or Aspin‐Welch t test, two‐sided). LCZ: lanoconazole, TPA: 12‐ O ‐tetradecanoylphorbol‐13‐acetate, KC: keratinocyte‐derived chemokine, MIP‐2: macrophage inflammatory protein‐2 and MPO: myeloperoxidase

Article Snippet: Other reagents used were as follows: TPA (Sigma‐Aldrich); PC (Tokyo Chemical Industry Co., Ltd); mouse CXCL1/KC Quantikine ® enzyme‐linked immunosorbent assay (ELISA) kit, mouse CXCL2/MIP‐2 Quantikine ® ELISA kit and mouse IFN‐γ Quantikine ® ELISA kit (R&D Systems Inc); and Fluoro MPO™ Fluorescent Myeloperoxidase Detection Kit (Cell Technology).

Techniques: Activity Assay, Fluorescence, Enzyme-linked Immunosorbent Assay, Comparison, Derivative Assay

Journal: Mycoses

Article Title: Anti‐inflammatory effect of lanoconazole on 12‐ O ‐tetradecanoylphorbol‐13‐acetate‐ and 2,4,6‐trinitrophenyl chloride–induced skin inflammation in mice

doi: 10.1111/myc.13034

Figure Lengend Snippet: Effect of antifungal agents on ear swelling of TPA‐induced irritant dermatitis in mice. Each antifungal agent (1%) or vehicle (acetone) was topically administered (20 µL/ear) immediately after TPA application. (A) The ear thickness was measured 6 h after the application of acetone or TPA. (B–D) MPO activity, KC content and MIP‐2 content in the 5‐mm‐diameter punch biopsy obtained 6 h after TPA application were measured by fluorescence and enzyme‐linked immunosorbent assay, respectively. Each column represents the mean ± standard error of the mean (n = 6). ** P < .01 compared with TPA alone (Aspin‐Welch t test or Student's t test, two‐sided); †† P < .01 compared with TPA (−) (Aspin‐Welch t test, two‐sided). (E–G) The relationships between the ear thickness and MPO activity, KC content and MIP‐2 content were analysed. Closed circle: LCZ, open square: LNF, open diamond: TBF and open triangle: AMO. AMO, amorolfine; KC, keratinocyte‐derived chemokine; LCZ, lanoconazole; LNF, liranaftate; MIP‐2, macrophage inflammatory protein‐2; MPO, myeloperoxidase; TBF, terbinafine; TPA, 12‐ O ‐tetradecanoylphorbol‐13‐acetate

Article Snippet: Other reagents used were as follows: TPA (Sigma‐Aldrich); PC (Tokyo Chemical Industry Co., Ltd); mouse CXCL1/KC Quantikine ® enzyme‐linked immunosorbent assay (ELISA) kit, mouse CXCL2/MIP‐2 Quantikine ® ELISA kit and mouse IFN‐γ Quantikine ® ELISA kit (R&D Systems Inc); and Fluoro MPO™ Fluorescent Myeloperoxidase Detection Kit (Cell Technology).

Techniques: Activity Assay, Fluorescence, Enzyme-linked Immunosorbent Assay, Derivative Assay

Journal: International Journal of Cancer. Journal International du Cancer

Article Title: Induction of proinflammatory mediators by CHI3L1 is reduced by chitin treatment: decreased tumor metastasis in a breast cancer model

doi: 10.1002/ijc.26379

Figure Lengend Snippet: CHI3L1 increases the expression of CCL2, CXCL2 and MMP-9. Macrophages from normal mice were cultured overnight with either 1 ng/mL or 5 ng/mL rmCHI3L1 alone (A,C,E) or in combination with LPS (1 μg/mL) (B,D,F) and cell-free supernatants were analyzed by ELISA for CCL2 (A–B) ; CXCL2 (C–D) or MMP-9 (E–F). N= 10 mice/group, (A–B) *p<0.0015, **p<0.0001, (CD)*p<0.0005, ** p<0.0002, and (E–F) *p<0.004, **p<0.0016.

Article Snippet: Cell culture supernatants and sera from control and mammary tumor bearers were analyzed for protein expression by ELISA for CCL2, CXCL2 and IFN-γ (BD Biosciences, San Jose, CA), MMP-9 and CHI3L1 (R&D Systems) according to the manufacturer's instructions.

Techniques: Expressing, Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: International Journal of Cancer. Journal International du Cancer

Article Title: Induction of proinflammatory mediators by CHI3L1 is reduced by chitin treatment: decreased tumor metastasis in a breast cancer model

doi: 10.1002/ijc.26379

Figure Lengend Snippet: Silencing CHI3L1 decreases secretion of proinflammatory molecules by macrophages. (A) In vitro treatment of LPS (1 μg/mL) stimulated macrophages from 5-week DA-3 tumor bearers with 50 nM CHI3L1 siRNA analyzed by qRT-PCR for CHI3L1 gene expression and protein expression as determined by ELISA (B). (C–E) Purified macrophages from 5-week DA-3 tumor bearers were cultured with 50 nM CHI3L1 siRNA or 50 NM nontarget siRNA in the presence of LPS and cell-free culture supernatants analyzed for CCL2 (C), CXCL2 (D) and MMP-9 (E). N = 5 mice/group, *p < 0.05, Student's t-test

Article Snippet: Cell culture supernatants and sera from control and mammary tumor bearers were analyzed for protein expression by ELISA for CCL2, CXCL2 and IFN-γ (BD Biosciences, San Jose, CA), MMP-9 and CHI3L1 (R&D Systems) according to the manufacturer's instructions.

Techniques: In Vitro, Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay, Purification, Cell Culture

Journal: International Journal of Cancer. Journal International du Cancer

Article Title: Induction of proinflammatory mediators by CHI3L1 is reduced by chitin treatment: decreased tumor metastasis in a breast cancer model

doi: 10.1002/ijc.26379

Figure Lengend Snippet: In vivo treatment with chitin decreases CHI3L1, CCL2, CXCL2 and MMP-9 expression in DA-3 tumor-bearing mice. (A) Serum from 2- and 5-week DA-3 tumor bearers either untreated or chitin treated (1 mg/mouse) was analyzed for CHI3L1 expression by ELISA. (B–C) Splenocytes (B) and splenic macrophages (C) from untreated or chitin treated 5-week DA-3 tumor-bearing mice were cultured overnight in the presence or absence of LPS (1 μg/mL) and cell-free supernatants were analyzed for CHI3L1 by ELISA. (D–F) Purified macrophages and T cells from spleens of 5-week DA-3 tumor-bearing mice, untreated, or chitin treated were cultured overnight with mitogens as described, and cell-free supernatants were analyzed by ELISA for CCL2 (D), CXCL2 (E) and MMP-9 (F). Data shown are the results of three independent experiments with N= 4 mice/group; *p<0.003, **p<0.002, Student's t-test

Article Snippet: Cell culture supernatants and sera from control and mammary tumor bearers were analyzed for protein expression by ELISA for CCL2, CXCL2 and IFN-γ (BD Biosciences, San Jose, CA), MMP-9 and CHI3L1 (R&D Systems) according to the manufacturer's instructions.

Techniques: In Vivo, Expressing, Enzyme-linked Immunosorbent Assay, Cell Culture, Purification